I have observed that prolonged culture periods are associated with increased cell attrition in my current system. Two non-exclusive explanations remain plausible: (1) stress intrinsic to the culture environment may reduce viability; and (2) developmentally programmed selection may account for part of the loss. At present, it remains unclear which factor predominates in my case.
Potential batch-to-batch variability further complicates interpretation, limiting my ability to draw definitive conclusions about optimal conditions or to fully validate the working hypothesis. Nevertheless, I can identify consistent trends under specific, well-controlled settings. For now, I will prioritize collecting more granular, quantitative readouts within the existing framework and defer exploratory alternatives to avoid confounding the analysis. My intent is to consolidate the observations and prepare a manuscript when the dataset is sufficiently validated.
The views expressed here are solely my own and do not represent those of my laboratory. No unpublished proprietary protocols or data are disclosed.
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